Datura stramonium plants on drug resistant pathogens isolated from clinical specimens dr. Antimicrobial activity of cotton and silk fabrics dyed. The antibacterial activity of the methanol extracts of the aerial parts of the datura innoxia and datura stramonium was investigated. The fine powder were collected into a sterile aluminum foil and kept in a cool dry place till further use. Datura stramonium was examined for its antimicrobial activity on some oral pathogens using methanol, chloroform, acetone and water as extractants. Antibacterial compounds in different species of datura a. Antimicrobial activity of cotton and silk fabrics dyed with datura stramonium jimson weed plant leaf extracts alex musinguzi department of manufacturing, industrial and textile engineering, school of engineering, p. Datura stramonium extract, zone of inhibition, antibacterial activity, antifungal activity, agar well diffusion method, minimum inhibitory concentration. The present study was aimed to screen the phytochemicals and to evaluate nematicidal activity of leaf of datura metel, datura innoxia and brugmansia suaveolens. The results of agar well diffusion assay indicated that the pattern of inhibition depends largely. Natural dyes and its antimicrobial effect smita bhuyan, dr. Antimicrobial activity of polyphenols and alkaloids in. Besides its hallucinogen activity, datura reported to have antimicrobial activity rajesh and sharma, 2002.
In vitro evaluation of datura innoxia thornapple for. Nematicidal activity of aqueous leaf extracts of datura. Phytoconstituents such as alkaloids, steroids, flavonoids, terpenoids, phenolic compounds, tannins, cardiac glycosides, anthroquinone glycosides, saponins and triterpenes were analyzed by qualitatively in aqueous leaf extracts of 1. Pdf antimicrobial property of datura leaf extract against methicillin. Paramedical college, dehradun248 991, india department of environmental science, graphic era university. Its diverse biological activities include antiasthmatic, antibacterial, antifungal, antiinflammatory, larvacidal. Nagesh malik1, samreen ahmed2 1,2 department of microbiology, v. Abstract datura stramonium is a plant of medicinal plant family solananceae. Datura, jimson weed datura stramonium, packet of 50. Zoology department, samrat prithviraj chauhan govt. Phytochemical analysis, antioxidant and in vivo biological. Its extracts show significant antimicrobial activity against staphylococcus aureus, proteus vulgaris, pseudomonas aeruginosa, escherichia coli, aspergillus niger and fusarium species 21. Richa bhardwaj, ankita yadav, pallavi sharma and ram avatar sharma, 2014. Abstract the phytochemical analysis and antimicrobial activities of in vitro grown callus and in d.
Joshi and kaur 20, worked on antimicrobial activity of ethanol, methanol and aqueous extract of d. Datura stramonium, crude extract, phytochemical screening, antimicrobial activity, paper disk diffusion method. Ethanolic extract showed more antibacterial activity than the aqueous extract. Callus induction and antimicrobial activities of callus. College of arts, science and commerce, chembur, mumbai 400071, india.
Datura stramonium the antimicrobial activity of the aqueous and ethanolic extract of the stembark of datura stramonium was investigation against staphylococcus aureus, salmonella typhi, shigella spp, eschericia coli, klebsiella pneumonia and neisseria gonorrhea. Datura stramonium, callus induction, antibacterial activity, antifungal activity, phytochemical analysis. Antimicrobial activity of latex of calotropis gigantea against pathogenic microorganisms an in vitro study gaurav kumar, loganathan karthik and kokati venkat bhaskara rao molecular and microbiology research lab, environmental biotechnology division, school of bio science and technology, vit university, vellore632 014, tamil nadu, india. Extraction and purification of antimicrobial proteins from. Pdf antimicrobial investigation of datura stramonium leaf. Comparative antimicrobial activity and phytochemical. Datura stramonium is commonly known as jimson weed and is widely used in phytomedicine to treat epilepsy, skin ulcers, etc as well as to kill the microbes associated with wound lesions.
Chemical tests and spectrophotometric methods were used for antioxidant activity. We are still open for nursery plant sales, and look forward to your live root and plant orders. Test organism the microorganism used for this study, bacterium. Introduction datura species can be found throughout the world, except the colder or arctic regions, it grows wild in all of the worlds warm and moderate region and it is very distributed in the temperate and tropical areas 18. Manjunath department of biochemistry, central college campus, bangalore university. The acceptance of traditional medicines as an alternative form of health care has led researchers to investigate the antimicrobial activity of medicinal plants 2, 3. Antimicrobial activities of methanolic extract of datura stramonium seeds material and methods. Its extracts show significant antimicrobial activity against staphylococcus aureus, proteusvulgaris, pseudomonasaeruginosa, escherichia coli, aspergillus niger and fusariumspecies. Phytochemical and antimicrobial property of datura stramonium 140 pestle. Datura stramonium and phytolacca dodecandra in different solvents. The extraction was carried out using trishcl buffer prepared from 50m tris and 0.
An extract of datura stramonium was screened for phytochemical analysis and antimicrobial assay against different bacterial species. Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas aeruginosa, agrobacterium tumefaciens and klebsiella. It is 60120 cm or more tall, branched, and pubescent plant. The general results observed consider the plant as natural source of antioxidants and phytochemical quality for. Introduction datura stramonium is an annual herb, with stem erect and spreading branches above. The aqueous extract of the plant displayed antioxidant activity of between 49. Antimicrobial activity of ethyl acetate and methanol extracts of leaves and flowers of datura stramonium was investigated against antibiotic.
Various parts of datura innoxia were examined for potential antibacterial activity by preparing their crude aqueous and organic extracts against gramnegative bacteria escherichia coli and salmonella typhi and grampositive bacteria bacillus cereus, bacillus subtilis and staphylococcus aureus. The phytochemical analysis of extracts were performed, and antimicrobial activity of aqueous and alcoholic extracts. Chemical constituents and pharmacological properties of. Comparative bacterial inhibition by bioactive extracts. Datura stramonium linn solanaceae grows as a wasteland weed 4, 5 mostly in the tropical and temperate areas of the world. These dried seeds were pulverized using sterile laboratory mortar and. Little or no antibacterial activity was found against escherichia coli and. Alkaloids, antibacterial activity, datura stramonium, gas chromatographymass specroscopy. Antibacterial and antifungal activity of different extracts of datura. Antimicrobial activity seed oil datura metel jebtm authors info r.
Antimicrobial activity of some common indian medicinal. Phytochemical screening and antimicrobial assessment of abutilon. In this study, crude extracts from fungal endophytes isolated from datura stramonium were evaluated for cytotoxic activity on two human cancer cell lines. Antimicrobial activity of carica papaya, piper nigrum and. Little or no antimicrobial activity was found against escherichia coli and psuedomonas aeruginosa. In vitro and in vivo gcms profile and antimicrobial activity of phytosterols of datura stramonium. Comparative antimicrobial activity and phytochemical analysis of datura stramonium l. The dried seeds of datura stramonium which were obtained from seed pods of datura plant. Joshi 11 23 1 2 3 department of chemistry, graphic era university, dehradun248 002, india department of biotechnology, doon p. The solvent used for extract preparation was methanol. Dried plant leaves of datura stramonium, carica papaya and fruits of piper nigrum.
Antimicrobial activity of some common indian medicinal plants against some selective human pathogen h. Comparative bacterial inhibition by bioactive extracts from datura stramonium and phytolacca dodecandra. Analysis of alkaloid phytochemical compounds in the. Endophytic fungi are a proven source of bioactive secondary metabolites that may provide lead compounds for novel drug discovery. Antibacterial activity of the plant extract and determination of minimum inhibitory concentration.
Leaves, seeds and roots contain the alkaloid daturine. Home shop datura, jimson weed datura stramonium, packet of 50 seeds, organic we at strictly medicinal seeds are dedicated to providing medicinal plants to the people. This study is conducted to investigate the antimicrobial property of proteins present in the seeds of datura stramonium. Leaf extract of datura stramonium shows strong antimicrobial activity against bacterial species like bacillus thuringiensis, pseudomonas. Aerial part mainly stem and bark of datura stramonium s aqueous and ethanolic extract opposite to eschericia coli. Datura stramonium solanaceae is an important medicinal plant from which tropane alkaloids, amino acids, tannin, phytic acids, carbohydrates have been isolated. Little or no antibacterial activity was found against escherichia coli and pseudomonas aeruginosa. It is common in the waste land, fields and gardens in baghdad district figure 1. The antibacterial activity reveals that the methanolic extract of datura stramonium seeds is highly active against both gram positive and gram. Antimicrobial activity of datura innoxia and datura. Antimicrobial property of datura leaf extract against. Comparative antimicrobial activity and phytochemical analysis of.
Phytochemical screening and antimicrobial assessment of. Datura stramonium is a natural source of antioxidants and phytochemical having antimicrobial activities. Comparative bacterial inhibition by bioactive extracts from datura. The present study was conducted to identify the preliminary phytochemical identification and antimicrobial investigation of leaves extracts of datura stramonium linn collected from eastern ethiopia. The main objective of this study was to evaluate the phytochemical composition, antioxidant, antifungal and insecticidal activities of aqueous and methanolic extracts. Evaluation of acute and subacute toxicity of alkaloids. The secondary metabolites of datura stramonium are highly effective against different types of diseases such as antdiabetic, antiviral, etc. The present study was aimed to investigate the phytochemical screening and antimicrobial assessment of adhatoda vasica, azadirachta indica and datura stramonium leaf extract. Antioxidant and antimicrobial properties of seed oil of.
The aim of the present study was to investigate the antimicrobial activity and phytochemical screening. Phytochemical and antimicrobial property of datura stramonium. Pdf antimicrobial investigation of datura stramonium. Antimicrobial activity of datura innoxia and datura stramonium. The aim of this study was to assess the compounds of alkaloids extracts from the leaves of datura stramonium, which can be the basis for the synthesis of new antibiotics. All datura plants contain tropane alkaloid such as hyoscyamine, scopolamine and atropine in the seeds and the flowers.
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